Simple temperature change makes gene-editing more efficient
By Bill Hathaway
December 8, 2017
Yale geneticists have expanded the potential uses of a groundbreaking gene-editing technology by modifying a single variable — temperature.
The CRISPR-Cpf1 gene-editing technology has proved to be highly efficient in mice but not in other model organisms. The Yale team showed that temperature is a key factor controlling Cpf1 activity and have optimized this technology allowing researchers to make targeted genetic changes in a host of model organisms such as zebrafish and the fruit fly Drosophila.
“With these optimizations, we can modify genomes of every other organism,” said lead author Miguel A. Moreno-Mateos.
“Now, we have a robust system able to efficiently edit regions in the genome not targetable by other CRISPR systems and this activity can be easy controlled just by changing the temperature.”